Journal: bioRxiv
Article Title: Maturing neutrophils of lower density associate with thrombocytopenia in Puumala orthohantavirus-caused hemorrhagic fever with renal syndrome
doi: 10.1101/2024.02.19.580937
Figure Lengend Snippet: ( A ) UMAP visualization of single live CD56 - /CD3 - /CD19 - /CD14 - /CD15 + /CD66b + cells, displaying LDGs (in blue) and PMNs (in red) in a concatenated format, enabling an in-depth examination of their phenotypic differences. ( B ) Multigraph color mapping depicting each fluorochrome’s Mean Fluorescence Intensity (MFI) for selected markers, including CD66b, CD11b, LOX-1, CD16, CD15, HLA-DR, and CD33. ( C ) Bar graph illustrating the proportion of PMNs and LDGs expressing CD16.
Article Snippet: Before cell staining, 1-2×10 6 cells were incubated with Fc receptor blocking reagent (BioLegend) for 5 min at RT, followed by a fixable dead cell stain (Invitrogen) and surface staining in the dark for 30 min at RT with the titrated fluorescently labeled anti-human monoclonal antibody cocktail: CD3 FITC (clone OKT3, catalog #: 11-0037-42) and CD66b PE-Cy7 (clone G10F5, catalog #: 25-0666-42) from Thermo Scientific; CD56 FITC (clone MEM-188, catalog #: 21270563) from ImmunoTools; CD19 FITC (clone HIB19, catalog #: 302206), CD33 APC (clone P67.6, catalog #: 366605), LOX-1 PE (clone: 15C4, catalog #: 358603), CD11b APC-Cy7 (clone ICRF44, catalog #: 301341) from BioLegend; CD16 BV421 (clone 3G8, catalog #: 562878), HLA-DR BV786 (clone G46-6, catalog #: 564041), and CD15 BV510 (clone W6D3, catalog #: 563141) from BD Biosciences.
Techniques: Fluorescence, Expressing